چکیده:
Background. The accumulation of excess triglyceride in the liver and a decrease in brown adipose tissue is related to
the fatty liver disease. Regular physical activity can take part in regulating fat oxidation and inhibiting fat
accumulation by creating and releasing some myokines. Nevertheless, the effectiveness of each training method in
this regard is still not certain. Objective(s). This study aims at comparing the effects of resistance and endurance
training on FNDC5 (the precursor of irisin myokine) gene expression in male rat liver tissues.. Methods. Fifteen
Wistar male rats (aged 10-12 weeks with an average weight of 331.8 ±63.09 gr) were used. After being taken to the
animals’ laboratory, the subjects were randomly assigned to three equal groups viz: control (N=5), endurance (N=5)
and resistance (N=5). For the purpose of tissue sampling, all these subjects were anesthetized 72 hours after the
experiment had been completed. The liver tissue was immediately removed and then quickly frozen in liquid
nitrogen and kept at a temperature of -80o C until RNA extraction. FNDC5 relative gene expression was identified
by Real-time PCR method. Results. Data analysis revealed a significant difference in FNDC5 gene expression
among the groups (P=0.008, Chi Square=31.791). No significant difference was observed between the resistance
training and endurance training groups in FNDC5 gene expression, though (P=0.959). Conclusion. It can be
established that both resistance and endurance training can similarly affect the production and secretion of exerciseinduced
myokines, including FNDC5 irisin, which can in turn protect against obesity and its impacts on the liver
tissue by stimulating the process of browning of white fat.
خلاصه ماشینی:
"The Effects of Resistance and Endurance Training on the Liver Tissue FNDC5 mRNA Gene Expression in Male Rats 1Amir Rashidlamir, 2Mohammad Hoseinzadeh, 3Leili Zeiaddini Dashtkhaki 1Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
This study aims at comparing the effects of resistance and endurance training on FNDC5 (the precursor of irisin myokine) gene expression in male rat liver tissues..
It can be established that both resistance and endurance training can similarly affect the production and secretion of exercise- induced myokines, including FNDC5 irisin, which can in turn protect against obesity and its impacts on the liver tissue by stimulating the process of browning of white fat.
Real-Time PCR of Liver FNDC5 Relative mRNA Expression in Control (C), Endurance -Training (END), Resistance – Training (RES) Groups, in male Rats.
One possible scenario concerning the effect of long-term resistance training on body composition is that these exercises create signals which activate PGC-1ɑ in the skeletal muscle and this, in turn, induces the expression of FNDC5.
(رجوع شود به تصویر صفحه) APPLICABLE REMARKS This study supports that both aerobic and resistance training can similarly increase FNDC5 gene expression in the liver tissue, which could be an explanation for the way they play a role in the process of browning of white fat, therefore reducing the fat in the liver tissue and improving the condition.
Effect of eight weeks’ resistance training on plasma irisin protein level and muscle FNDC5 and adipose tissue UCP1 genes expression in male rats."