چکیده:
Investigating the effect of salinity on safflower hypocotyl callus, it was shown that the
salinity positively impacted on callogenesis of both sensitive and resistant types in both light
and darkness conditions; the increased NaCl concentrationled to increased callus index and
better calluseswere formed. Measuring free proline content in callus of safflower, it was
indicated that the increased salt concentration in culture medium led to increased
accumulated proline in sensitive varieties and decreased proline content in resistant varieties
which are under salt stress. The polyphenol oxidase enzyme activity increased largely with
increasing of salinity; this increase was observed in both resistant and sensitive varieties of
safflower and there was no significant difference between varieties in light and darkness
situations. Comparing sensitive and resistant varieties, it was shown that more enzymes
were produced in sensitive variety by stress. This study investigated the effect of different
NaCl (180, 120, 60, and zero mM) treatments oncallogenesisrate, qualitative and
quantitative measurements of polyphenol oxidase enzyme activity, and proline content in
calluses of safflower hypocotyl in MS medium. The hormonal ratio of li mg =0.2Kin
و li mg 2 =2, 4 – D was used to study the effect of salinity on callogenesis of hypocotyl of
resistant and sensitive varieties of safflower in MS medium. The findings showed that the
different hormonal treatments (in terms of concentration) have different effects on growth
and appearance of calluses.
خلاصه ماشینی:
"Studying free proline content of calluses which are created in safflower hypocotyl in different concentrations of NacL Komeil Torabi MSc. Of biology, Ministry of Education, Zanjan, Iran.
Original Article: Received 05 Jan. 2017 Accepted 08 March 2017 Published 26 May. 2017 ABSTRACT Investigating the effect of salinity on safflower hypocotyl callus, it was shown that thesalinity positively impacted on callogenesis of both sensitive and resistant types in both light and darkness conditions; the increased NaCl concentrationled to increased callus index and better calluseswere formed.
Measuring free proline content in callus of safflower, it was indicated that the increased salt concentration in culture medium led to increased accumulated proline in sensitive varieties and decreased proline content in resistant varieties which are under salt stress.
This study investigated the effect of different NaCl (180, 120, 60, and zero mM) treatments oncallogenesisrate, qualitative and quantitative measurements of polyphenol oxidase enzyme activity, and proline content incalluses of safflower hypocotyl in MS medium.
Discussion and findings Measuring proline content in different (NaCl) concentrations showed that in most varieties of safflower, the increased NaCl concentration led to decreased proline content compared to control group.
The results of measuring values of free proline in calluses of safflower hypocotyl indicate that the proline content differed in resistant and sensitive varieties in presence of salt (NaCl); increasing of salt concentration in culture medium leads to increase of accumulated proline in sensitive variety (537-PI) and decrease of proline content in resistant variety (5151-LRV)."