چکیده:
During intense physical activity, tissues such as the liver, kidneys, and intestines experience hypoxia due to exercise-induced anemia, which ultimately leads to an increase in free radicals. The aim of this study was to investigate the effect of eight weeks of endurance training on levels of glutathione peroxidase (GPX) and malondialdehyde (MDA) in the gastrocnemius muscle, heart, and liver tissues of male Wistar rats. 12 male Wistar rats (mean weight 234.47±97.25 grams and age eight weeks) were randomly divided into two groups: control (n=6) and intense endurance training (n=6) after one week of familiarization. Endurance training lasted for eight weeks and included five sessions per week on a rodent treadmill. The speed and duration of running in the first week of the research were 10 meters per minute and 30 minutes, respectively, and in the last week reached a speed of 35 meters per minute (equivalent to 80 to 85 percent Vo2 max) and a time of 70 minutes. The results of the independent t-test showed that intense endurance training significantly reduced the levels of glutathione peroxidase in heart tissue (P=0.001) and liver tissue (P=0.001) compared to the control group. In addition, levels of malondialdehyde in heart tissue (P=0.03) and liver tissue (P=0.045) were significantly increased in the training group compared to the control group. The findings of the present study indicate different oxidative responses of various tissues to similar exercise, so that intense endurance training puts heart and liver tissue under more oxidative stress than gastrocnemius muscle.
خلاصه ماشینی:
The aim of the present study was to investigate the effect of eight weeks of endurance training on levels of glutathione peroxidase (GPX) and malondialdehyde (MDA) in the gastrocnemius muscle, heart, and liver tissues of male Wistar rats.
However, some studies show that high-volume endurance training, commonly used by elite athletes, reduces the efficiency of the antioxidant system, increases the production of free radicals in skeletal muscle and other active tissues, and ultimately causes oxidative stress (٨).
Therefore (١٦, ١٥) this study examines the capacity of the last line of antioxidant - antioxidant and lipid peroxidation of the gastrocnemius muscle, heart, and liver of male Wistar rats after eight weeks of intense endurance training.
(2015) showed that six weeks of continuous exercise (speed of 20 meters per minute (equivalent to 55% max Vo2) (18,17) for 20 minutes a day and five days a week) increased the antioxidant enzyme glutathione peroxidase and did not change malondialdehyde levels in heart tissue (15).
The present study showed that there is no significant difference in the activity level of glutathione peroxidase enzyme and malondialdehyde levels in the gastrocnemius muscle between the intense endurance training group and the control group.
In conclusion, the results of this study showed that the oxidative response of different tissues to similar exercise activity can vary, such that intense endurance activity (equivalent to about 80 to 85% Vo2 max) for eight weeks leads to a decrease in the levels of antioxidant enzymes in the heart and liver; while, it causes no change in skeletal muscle.
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