چکیده:
Introduction: Diabetes mellitus is a group of metabolic disorders that are characterized by a decline in insulin secretion, insulin malfunction or both. Visfatin is a recently discovered protein that is expressed and secreted in visceral fat tissue. Elevated plasma visfatin levels have been reported in humans with type 2 diabetes mellitus. The purpose of this study was to investigate the effect of a single bout of aerobic exercise on visceral adipose tissue visfatin gene expression in a diabetic rat model.Material and Methods: Diabetes was induced by streptozotocin (50 mg/kg) injection in a single dose administered to twenty male Wistar rats (6 – 8 weeks old, 165 ±5 grams weight). The rats were randomly divided into four groups, each comprising five rats; one control group and three exercise groups. The acute aerobic exercise bout required the three exercise groups to complete a single session of running for 50 minuteson a treadmill at a velocity of20m.min-1. Following the treadmill run, one of the exercise groups wereanesthetized immediately after the exercise, the second one 4h -after exercise and the last group were anesthetized 24h post exercise. Tissue samples were collected immediately after the animals were anesthetized. To determine the relative levels of gene expression, semi-quantitative RT-PCR was performed.Results: The results showed a significant increase in the expression of visfatin in the 4h and 24h postexercise groups compared with the control group (P<0.05).Discussion and Conclusion: In summary, exercise can improve the visceral fat tissue levels of visfatin mRNA expression. The increase in the visceral fat tissue visfatin mRNA suggests that visfatin in visceral fat tissue could possibly play an important role in glucose metabolism and act as a paracrine messenger.
خلاصه ماشینی:
"The purpose of this study was to investigate the effect of a single bout of aerobic exercise on visceral adipose tissue visfatin gene expression in a diabetic rat model.
Key words: Aerobic exercise, Type 2 diabetes, Visfatin Introduction A correlation between fat accumulation in visceral adiopose tissue,insulin resistance, obesity, and cardiovascular disease is well documented.
In the present study, we investigated the effect of a single session of aerobic exercise on visfatin mRNA levels in rat visceral adipose tissue[12].
Using a suitable post hoc test revealed a higher and significant visfatin gene expression in visceral adipose tissue 4 and 24 hours after the accomplishment of an acute aerobic exercise bout (Pvisceral adipose tissue visfatin mRNA increased in response to exercise compared with control group (Fig. 1)).
After Fukuhara et al (2005) showed the role of visfatin in glucose metabolism, other researchers investigated the association of type 2 diabetes, with insulin secretion and sensitivity as well as its relationship with other adipocytokines like adiponectin.
As previously mentioned, the present study is the first published research that has studied visceral adipose tissue visfatin gene expression in response to a single exercise session in diabetic subjects (animal and human).
The findings of this study showed a significant increased in the gene expression of visfatin in response to aerobic activity in diabetic rats 4 hrs and 24 hrs after exercise.
However, it is likely that changes in plasma visfatin in terms of its tissue expression is influenced by several factors such as decrease or increase in blood glucose, insulin, weight loss, body mass index, caloric restriction and fat-rich food."