چکیده:
Purpose: Nesfatin-1 is a protein derived from a precursor molecule of the nucleobindin-2 gene. Nesfatin-1 has been suggested to act as a novel inhibitor and a potential regulator of food intake and body weight. It has been reported that changes in proteins and peptides that are released from hypothalamus and adipose tissue are related to cardiovascular diseases (CAD). Therefore, the aim of this study was investigate the effects of eight weeks of aerobic training on nesfatin-1/nucleobindin-2 expression, plasma concentration, and plasma highdensity
lipoprotein (HDL-C) in female rats (Liver, small intestine and kidney tissues).
Material and Methods: In this study ten Wistar rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned into control and training groups. Training group was given exercise on a motor-driven treadmill at 25 m/min (0% grade), 60 min/day, 5 days/week, for eight weeks. 72 hours after the last training session and after four hours of fasting, rats were sacrificed. Liver, small intestine and kidney were excised, total RNA was extracted, and after PCR procedure, quantitative real-time RT-PCR was performed. Plasma was also collected for plasma variable measurement. Data are expressed as means ± SEM and the significance level was set at P<0.05.
Results: Exercise-induced increases in liver (P<0.001), small intestine (P<0.001) and kidney (P<0.019) nesfatin-1/NUCB2 expression were observed. A significant up-regulation in Plasma HDL-C concentration in the training group was also spotted (P<0.003) in comparison to the control group. Data analysis revealed no significant differences in plasma nesfatin-1 concentration between the study groups (P<0.093). Discussion and Conclusion: Tissue nesfatin-1/NUCB2 mRNA expression and plasma HCL-C concentration were affected (up-regulated) by physical exercise while plasma nesfatin-1 levels remained unchanged.
خلاصه ماشینی:
"Effects of Aerobic Training on Tissue Nesfatin-1/Nucleobindin-2 mRNA, Plasma Nesfatin-1 and High-density Lipoprotein Concentration in Female Rats Abbass Ghanbari-Niaki1, Saleh Rahmati-Ahmadabad2*, Zarbakht Ansari-Pirsaraei3 1 Department of Physical Education and Sport Science, University of Mazandarn, Baboulsar, Iran.
Therefore, the aim of this study was investigate the effects of eight weeks of aerobic training on nesfatin-1/nucleobindin-2 expression, plasma concentration, and plasma high- density lipoprotein (HDL-C) in female rats (Liver, small intestine and kidney tissues).
Expression of nesfatin-1 and its levels in serum/plasma could be affected by fasting and refeeding [3, 15, 16], restraint stress [17, 18], abdominal surgery [4], a high-fat diet [7, 19], and a glycaemic state [20] but no researches have examined the effect of exercise on tissue Nesfatin-1/NUCB2 gene expression.
Thus, the current study aimed to investigate the effects of eight weeks of aerobic training on nesfatin-1/nucleobindin-2 expression in female rats’ tissues including Liver, small intestine and kidney.
Results Data analysis revealed a significant difference in liver relative mRNA expression of nesfatin-1/NUCB2 at the end of treadmill running program (F= 273.
Data analysis revealed a significant difference in intestine relative mRNA expression of nesfatin- 1/NUCB2 at the end of treadmill running program (F=30.
In summary, this investigation studied the effects of eight weeks of endurance training on relative tissue expression, plasma concentration of nesfatin- 1, and plasma HDL-C concentrations in female rats.
The main finding of the present study was the higher and significant relative expression of nesfatin-1/ NUCB2 in the liver, small intestine and kidney tissues of female rats, following a treadmill running program."