Abstract:
Background. SIRT3 is one of the members of the Sirtuin deacetylase protein family which is linked to the longevity of human being and is used as an important therapeutic and diagnostic marker in illness and aging.
Objectives. The aim of this study was studying the effect of continuous swimming training on some biomarkers of longevity in slowtwitch (SOL) and fast-twitch (EDL) muscles of adult male rats. Methods. 20 healthy male, 5 months old rats were divided into two groups: control (n = 10) and exercise (n = 10) groups. Continuous swimming training was performed 5 days a week for 8 weeks, including 30 minutes of workout without adding weights in the first week to 60 minutes of workout, adding weights by 3% of the body weight in the eighth week. Twenty four hours after the last exercise session, SOL and EDL muscles were removed and the changes in variables (PGC-1α, SIRT3, and GSH: GSSG) were measured by ELISA method.
Results. The results showed that conducting 8 weeks of continuous swimming training significantly increased PGC-1α and SIRT3 levels in slow-twitch (SOL) and fast-twitch (EDL) muscles (p<0.05). There was no significant difference in the aging index (GSH: GSSG) of the SOL and EDL groups compared to the control group (p>0.05).
Conclusion. According to the findings of the present study, the implementation of continuous swimming exercises can improve the PGC-1α and SIRT3 proteins, which are biogenesis mitochondrial and life span biomarkers in slow- and fast-twitch muscles.
Machine summary:
Continuous Swimming Training Arises a Remarkable Effect on Some Longevity Biomarkers in Rat Skeletal Muscles 1Fatemeh Heiat, 1Mohsen Ghanbarzadeh*, 1Rouhollah Ranjbar, 2Manzarbanoo Shojaeifard 1Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
The results showed that conducting 8 weeks of continuous swimming training significantly increased PGC-1α and SIRT3 levels in slow-twitch (SOL) and fast-twitch (EDL) muscles (p < 0.
According to the findings of the present study, the implementation of continuous swimming exercises can improve the PGC-1α and SIRT3 proteins, which are biogenesis mitochondrial and life span biomarkers in slow- and fast-twitch muscles.
Therefore, considering the importance of delay the aging process and degenerative diseases caused by it in order to improve the quality of life, the researchers, with the design and implementation of this research, answered the question to what extent the Continuous Swimming Training can stimulate the mitochondrial biogenesis and increase the SIRT3 activity and finally suppresses ROS and improves the aging index in two types of slow-twitch muscle (SOL) and fast-twitch (EDL) muscles of adult male rats.
To prepare the tissue samples, a combination of Ketamine (60-40 mg/kg body weight) and Xylene (15-5 mg/kg body weight) were injected intraperitoneally and following the rats anesthesia, SOL and EDL muscles were removed in order to measure the amount of mitochondrial biogenesis proteins (PGC-1α, SIRT3) and GSH and GSSG (reduced and oxidized glutathione).